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KMID : 1011620210370010081
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Korean Journal of Food and Cookey Science
2021 Volume.37 No. 1 p.81 ~ p.92
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Comparative Analysis of the Flavonoid Content of Korean Ginger(Zingiber officinale Rosc.) Leaves and Stem by Solvent Extraction using UPLC-DAD-QTOF/MS
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Nam Dong-Geon
Lee Su-Ji
Im Jeong-Yeon
Hwang Kyung-A
Choe Jeong-Sook
Im Pu-Reum
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Abstract
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Purpose: To investigate the main phenolic compound in Korean ginger (Zingiber officinale Rosc.) leaves and stem by isolating the flavonoids content through solvent extraction using UPLC-DAD-QTOF/MS.
Methods: The flavonol glycosides were extracted using ethanol and hot-water. Thirteen flavonol glycosides were identified by a comparison of the ultraviolet and mass spectra with data from a chemical library and published literature.
Results: Among these, novel compounds quercetin and kaempferol glycosides [M+H]+: quercetin 3-O-robinobioside-7-O-rhamnoside [m/z 757.2189], quercetin 3-O-rutinoside-7-O-rhamnoside [m/z 757.2184], quercetin 3-O-galactoside-7-O-rhamnoside [m/z 611.1623], quercetin 3-O-glucoside-7-O-rhamnoside [m/z 611.1617], quercetin 3-O-robinobioside[m/z 611.1621], quercetin 3-O-rutinoside [m/z 611.1608], quercetin 3-O-glucoside [m/z 465.1039], kaempferol 3-O-robinobioside-7-O-rhamnoside [m/z 741.2239], kaempferol 3-O-rutinoside-7-O-rhamnoside [m/z 741.2242], kaempferol 3-O-galactoside-7-O-rhamnoside [m/z 595.1667], kaempferol 3-O-robinobioside [m/z 595.1663], kaempferol 3-O-rutinoside [m/z 595.1660], kaempferol 3-O-glucoside [m/z 449.1088] were newly confirmed from the ginger leaves and stem. In particular, quercetin 3-O-glucoside (isoquercitrin) and quercetin 3-O-rutinoside (rutin) were confirmed as the major compounds in flavonols. Ethanol extraction of the ginger leaves and stem(213.47±2.60 and 59.88±0.63 mg/100 g dry weight) showed a higher flavonoid content than hot-water extracts (27.48±0.19 and 7.13±0.04 mg/100 g dry weight).
Conclusion: This study has shown that, the use of ethanol in the solvent extraction process can significantly increase the flavonoid content obtained from ginger leaves and stem. The flavonoids in Korean ginger could serve as a potential source of natural antioxidants for use in various industrial applications.
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KEYWORD
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quercetin 3-O-rutinoside, Zingiber officinale Rosc., leaf, stem, UPLC-DAD-QTOF/MS
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